Iron Utilization Spectrum of Vibrio alginolyticus ATCC17749

Abstract

Iron is most essential micro-nutrient element that is required by microorganism including vibrio species. The mechanism of iron acquisition is complex by its existence in nature, insoluble ferric complexes and its high binding affinity by some proteins, specialized for iron binding protein in the host. Vibrio species have evolved various iron transport systems, permit bacteria to uptake iron from the growing medium. The present study is aimed to identification of putative iron transport genes, iron source utilization. The annotations of whole genome of V. alginolyticus ATCC 17749 analyzed revealed two putative iron transport: iron-siderophore transport system and heme and hemin transport system. Genes of iron transport system are present on both chromosomes in V. alginolyticus ATCC 17749 and encode ferric or ferrous iron transporter, protein for biosynthesis and transport of iron chelator, and unambiguous receptor for various types of chelated iron. The iron transport systems in V. alginolyticus, which covered approximately 1 % of the genome, is a quietly similar percentage of V. cholerae genome responsible for iron transport systems. It utilized heme, hemin ferrous sulfate and ferrioxamine in iron depleted growing environment. All data put together and analysis recommended that V. alginolyticus ATCC 17749 utilized ferrisiderophore and hemin derivative iron sources in iron depleted environment. These studies need to be further confirmed mutant library construction and screening of several gene specific mutants.